Entering edit mode
11 months ago
bioinfo223
▴
10
Hello,
I'm new to bioinformatics and I have a question about using MCScanX for a particular species. I have the genomic protein sequences and a GFF file for this species. However, after running BLASTP, I noticed that the IDs in the BLASTP results correspond to protein IDs, while the IDs in the GFF file represent gene IDs. As a result, when I use MCScanX, I'm getting zero synteny. Can you please advise me on how to resolve this issue? Thank you.
diamond blastp -e 1e-5 -p 30 -q v1_protein.faa -d GCF.aa -a augustus.aa.vs.self -b8 -c1
x.blast
NP_0013471.1 NP_0013471.1 100 166 0 0 1 166 1 166 2.30e-116 327
NP_0013471.1 XP_0425915.1 78.3 166 32 1 1 166 1 162 1.51e-86 252
x.gff
chr1 gene-LOC1226577 8679 12187
chr1 gene-LOC1226575 2966 7093
chr1 gene-LOC1092258 25095 34175
chr1 gene-LOC1090128 35304 50351