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5 months ago
Mark
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I'm doing a lot of de novo assemblies of rare eukaryotes and inside my assembly pipeline there are a lot of assembly algorithms that require the ploidy level of the organism. I have a program that estimates the ploidy level of the organism from the raw sequencing data based on grouping the reads found at the telomere ends of the organism but in order for this program to work it needs as input the organism's telomere repeat sequence. Is there a program that can just predict what the organisms telomere repeat sequences are just from the raw sequencing data?
After a cursory google search I discovered this database which might be of use to your project?
https://academic.oup.com/nar/article/52/D1/D311/7246534
Thank you for that. That's very useful but unfortunately it doesn't fully resolve my problem because a lot of the organisms I work with are bioprospected and have never been characterized so there's no genetic information for them to refer to. I would need a software to also help predict telomeres for organisms that aren't in the database.