root@86-empty:/data/soyeon/tool/cufflinks-2.2.1.Linux_x86_64# ./cuffquant --no-update-check -o /data/soyeon/20240315_RNA_FPKM/cxb -p 10 --library-type fr-firststrand /data/soyeon/reference_genome/hg38.refGene.gtf /data/soyeon/20240315_RNA_FPKM/mapping_bam/2+Aligned.sortedByCoord.out.bam [11:31:41] Loading reference annotation. [11:31:51] Inspecting maps and determining fragment length distributions.
The issue arises during the "Inspecting maps and determining fragment length distributions." stage of the "cuffquant" command, where the process takes a considerable amount of time. It takes more than an hour. It originally took about 7 minutes per sample, but it is strange that it now takes over an hour.
Does it take this long? Somebody help me, please...
