Extract Umi sequence to readname
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8 months ago
Smilesky • 0
@BH04640:4:22CNJJLT3:5:1101:27120:1048 1:N:0:CAAGGTGA+AGTCGCGA
GNACTGTTATCTATTGAAGGACATGTGCATTACCTTATACAGGAAGCTACTGATGAAAACTTACTATGCCAGATGTATCTTGGTTGGACTCCATATATGTGAAATGAAATTATGTAAAAGAATATGTTAAAGTAGAGATCGGAAGAGCAC
+
I#IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII          

My sequence looks like this. I am trying to extract the umi sequence using FGBIO - FastqToBam tool. can anyone tell me How to Extract the UMI in ubam. Because when i run the FastqToBam the umis are not extracted

BH04640:4:22CNJJLT3:5:1101:27009:1048:CAAGGTGA+AGTCGCGA 4   *   0   0   *   *   0   0   CNAGTAGCTAGAGTGGTGGCACATGCCTGTAGTCCCAGCTACTCGGAAGGCTGAGGTGGGAGAATCACTTGAACTCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGCCTGGGAGACAGAGCAAGACTCTGCCT  I#IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII  RG:Z:A.

I also want to know the coressponding read structure for the sample given

Sequence FgBio UMI • 368 views
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Can you post your entire FastqToBam command?

Are you telling the program the structure of your UMI's as noted here: http://fulcrumgenomics.github.io/fgbio/tools/latest/FastqToBam.html with --read-structures option.

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