How to perform GO enrichment analysis for assembled sequence ?

Question

[Sony]

Hello everyone,

I have an assembled sequence of Brassica. I already blast search assembled contigs against Plant SwissProt protein database. The command that I used:

blastx -query clean.fasta -db uniprot_sprot_plants_db -outfmt 6 -evalue 1e-5 -out blast_masurca_plantswissprot.tsv -num_threads 4

I want to perform the GO enrichment analysis, annotated sequences with Gene Ontology. I have read the manual of Blast2Go, but I have so many problems. Is there any possible program using the command line to do that, and can generate the results via charts and graphs to easily visualise?

Thank you.

[Istvan Albert]

you say you have so many problems with Blast2Go, what are these problems?

also make you understand that what you seem to want is a functional annotation not an GO enrichment analysis