POD5 files

Question

[marco.barr]

Hello everyone, I'm wondering if there's a way to extract the reads located on the positive strand from pod5 files. Which commands among pod5 subset, filter from pod5 tool can I use? Or should I work directly from the fastq files? Thanks for your help.

[GenoMax]

If you know which strand is "positive" then you should be able to get the reads from alignments using something like Samtools View: Only Forward Or Reverse Strand