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7 months ago
Jiang
•
0
I have finished my Eukaryotic genome annotation using augustus. I have got the gff3 file. It seems that I need to extract information from the gff3 file for the downstream functional annotation. But I don't know how to do it in linux. Could anyone provide some suggestions?
For genomic feature extraction I recommend AGAT or gffread:
https://agat.readthedocs.io/en/latest/tools/agat_sp_extract_sequences.html
https://github.com/gpertea/gffread
you should be more specific as to what you are trying to do.
in general parsing a GFF file, while error prone and annoying it is reasonably straightforward,
look at your file with a text editor and identity what steps are you trying to achieve aka which field of which region should be extracted into what foramt