Software to separate reads from different individuals
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6 months ago
njornet ▴ 20

I have a sample of a mixture of DNA from two different individuals (human) and I want to separate the reads sequenced by Nanopore (long reads) from each person. Does it exist a software that can do this or do I have to implement it myself?

Thank you!

software development nanopore • 413 views
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Were these samples barcoded when they were run. If not then you won't be able to do much.

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No... the extracted sample is already a mix so no way to barcode

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If you have independent information available (e.g. genotype data for both individuals) then you may be able to assign reads based on the presence of SNP.

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