barcode of TCR-sequencing

Question

[yueli7]

Hello,

I have paired-end TCR-sequencing reads. After removed adapter and filtered low quality reads, I got the clean reads.

I will do the analyze by using mixcr.

I know each TCR-sequencing corresponding to another scRNA-sequencing.

My question is: Do I need to remove barcode?

Thank you in advance for great help!

Best,

Yue

Answer 1

[mizraelson]

Hi, What protocol did you use for TCR-seq library preparation? Generally speaking, there is no need to remove barcodes, as MiXCR can work with barcoded data. However, you need to use the correct preset. You can find more information on presets here:

https://mixcr.com/mixcr/reference/overview-built-in-presets/

Sincerely, Mark