PseudobulkExpression.Seurat error
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Entering edit mode
7 months ago
sooni ▴ 20
The following error occurred in PseudobulkExpression.Seurat(object = object, assays = assays,:
   None of the requested assays are present in the object.

The above error occurs. Below is the code I ran:

cts <- AggregateExpression(seurat_from_cds,
                           group.by = c("Cell.Type", "Samples"),
                           assays = "originalexp",
                           slot = "Counts",
                           return.seurat = F)

How can I solve this error?

Thank you for help!

Seurat pseudo-bulk • 399 views
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Entering edit mode

None of the requested assays are present in the object

Does the assay originalexp exist in your object seurat_from_cds ?

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