Enhanced Volcano-Cut off

Question

[odi]

When you write the enhanced volcano code; using this as an example...

EnhancedVolcano(filtered_mutant1,
                lab = filtered_mutant1$gene,
                x = 'avg_log2FC',
                y = 'p_val',
                xlab = bquote(~Log[2]~ 'fold change'), title = "Cluster 1: BGH vs WT", 
                pCutoff = 0.05,
                FCcutoff = 0.25)

I am just wondering in this case, if FCcutoff is 0.25; does that mean that the log2fc cutoff is = 0.25 or does that mean that FC = 0.25?

Thank you for your assistance and this great package

Answer 1

[marco.barr]

from the documentation it seems that it reports limits for the value of log2 fold-change in an absolute sense.

FCcutoff: Cut-off for absolute log2 fold-change. Vertical lines will be drawn at the negative and positive values of log2FCcutoff