Entering edit mode
6 months ago
Peter Chung
▴
210
hello I have an error when I ran the nextflow sarek pipeline:
nextflow -bg run nf-core/sarek -r 3.4.0 -params-file params_samplesheet_F52_3.json -profile docker &
Workflow execution completed unsuccessfully!
The exit status of the task that caused the workflow execution to fail was: 125.
The full error message was:
Error executing process > 'NFCORE_SAREK:SAREK:BAM_BASERECALIBRATOR:GATK4_BASERECALIBRATOR (120236)'
Caused by:
Process `NFCORE_SAREK:SAREK:BAM_BASERECALIBRATOR:GATK4_BASERECALIBRATOR (120236)` terminated with an error exit status
(125)
Command executed:
gatk --java-options "-Xmx3276M -XX:-UsePerfData" \
BaseRecalibrator \
--input 120236.converted.cram \
--output 120236_chr1_122026460-124977944.recal.table \
--reference Homo_sapiens_assembly38.fasta \
--intervals chr1_122026460-124977944.bed \
--known-sites dbsnp_146.hg38.vcf.gz --known-sites Mills_and_1000G_gold_standard.indels.hg38.vcf.gz --known-sites
Homo_sapiens_assembly38.known_indels.vcf.gz \
--tmp-dir . \
cat <<-END_VERSIONS > versions.yml
"NFCORE_SAREK:SAREK:BAM_BASERECALIBRATOR:GATK4_BASERECALIBRATOR":
gatk4: $(echo $(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*$//')
END_VERSIONS
Command exit status:
125
Command output:
(empty)
Command error:
docker: Error response from daemon: failed to create endpoint nxf-02MAUnCuusW7YHwLnsBbzWSA on network bridge: failed to add the host
(vethe58dde8) <=> sandbox (veth83d3dac) pair interfaces: cannot allocate memory.
time="2024-05-21T11:53:49+08:00" level=error msg="error waiting for container: context canceled"
Work dir:
/data/hhgphk/runF52/work/00/8748af7adb7ffb4cbacc79d0fedbca
Tip: you can try to figure out what's wrong by changing to the process work dir and showing the script file named `.command.sh`
I tried to add the memory limit to docker file:
sudo nano /etc/default/docker
DOCKER_OPTS="--default-memory-limit=32768m --default-memory-swap=65536m"
My docker and nextflow version: Docker version 20.10.24, build 297e128
N E X T F L O W
version 23.10.0 build 5889
created 15-10-2023 15:07 UTC (23:07 HKST)
cite doi:10.1038/nbt.3820
http://nextflow.io
But it still does not work. I am new to nextflow, can anyone give me advice. Thanks.
you should ask : https://github.com/nf-core/sarek/issues/