Hi all,
I performed VIPER analysis to obtain transcription factor activity normalized enrichment scores (NES
) corresponding my samples. I would like to plot a heatmap to show the difference in NES
for multiple transcription factors. However, I have come across this problem whereby the range of NES
goes from -15 to 15, but the NES difference
between my two groups of samples are around 1 to 2. As a result, when I plot a heatmap, the activity differences between the groups are not obvious.
Is there a way to rescale the NES
where it can be justified? I have thought about calculating the NES zscore
or log normalization of the absolute NES score
. Are these justifiable? Thanks.