ERROR with Maker annotation: The assumed RepeatMasker installation directory /opt/home/sony/miniconda3/envs/MAKER2/share/RepeatMasker does not appear to be correct. E.g it does not contain a 'Lib
0
0
Entering edit mode
6 months ago
Sony ▴ 10

Hi everyone,

I am going to do the gene prediction and annotation for my rice genome assembled sequence. I installed RepeatModeler via conda and built and Made a repeat library for my rice-assembled genome fasta file. I also installed MAKER version 3.01.03 via Conda enter link description here Then, I run MAKER as in the tutorial enter link description here

These are commands that I used to run MAKER:

maker -CTL  
export ZOE=/opt/home/sony/miniconda3/envs/MAKER2/bin/snap 
export AUGUSTUS_CONFIG_PATH=/opt/home/sony/miniconda3/envs/MAKER2/bin/augustus/config

I run MAKER with a script

mpiexec -n 4 maker -base En_Pan_MaS_R1 maker_opts.ctl maker_bopts.ctl maker_exe.ctl
bash ./round1_run_maker.sh 2>&1 | tee round1_run_maker.log

However, I encountered this error:

setting up GFF3 output and fasta chunks
setting up GFF3 output and fasta chunks
doing repeat masking
running  repeat masker.
#--------- command -------------#
Widget::RepeatMasker:
cd /tmp/maker_kG9F4R; /opt/home/sony/miniconda3/envs/MAKER2/bin/RepeatMasker /opt/data/sony/thesis/Repeat_Oi_Nip/MaSuRCA/En_Pan_MaS_R1.maker.output/En_Pan_MaS_R1_datastore/CC/6F/scf7180000020707//theVoid.scf7180000020707/0/scf7180000020707.0.rice.rb -species rice -dir /opt/data/sony/thesis/Repeat_Oi_Nip/MaSuRCA/En_Pan_MaS_R1.maker.output/En_Pan_MaS_R1_datastore/CC/6F/scf7180000020707//theVoid.scf7180000020707/0 -pa 1
#-------------------------------#
doing repeat masking
doing repeat masking
The assumed RepeatMasker installation directory
    /opt/home/sony/miniconda3/envs/MAKER2/share/RepeatMasker
does not appear to be correct.  E.g it does not
contain a 'Libraries' or 'Matrices' subdirectory.
This can occur if hard links are used to invoke
this script.
ERROR: RepeatMasker failed
--> rank=3, hostname=hpz6
ERROR: Failed while doing repeat masking
ERROR: Chunk failed at level:0, tier_type:1
FAILED CONTIG:scf7180000020707

ERROR: Chunk failed at level:2, tier_type:0
FAILED CONTIG:scf7180000020707

examining contents of the fasta file and run log



--Next Contig--

#---------------------------------------------------------------------
Now starting the contig!!
SeqID: scf7180000020709
Length: 2066
#---------------------------------------------------------------------


setting up GFF3 output and fasta chunks
doing repeat masking

I already check the presence of 'Libraries' or 'Matrices' in /opt/home/sony/miniconda3/envs/MAKER2/share/RepeatMasker , these are available.

How can I solve these error? This is my first time doing gene prediction and annotation. Thank you everyone for any suggestions.

RepeatMasker. MAKER. annotation. • 650 views
ADD COMMENT

Login before adding your answer.

Traffic: 2283 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6