Long-read single-cell transcriptomics (scRNA-Seq) is revolutionizing the way we profile heterogeneity in disease. Traditional short-read scRNA-Seq methods are limited in their ability to provide complete transcript coverage, resolve isoforms, and identify novel transcripts. The scRNA-Seq protocols developed for long-read sequencing platforms overcome these limitations by enabling the characterization of full-length transcripts. Long-read scRNA-Seq techniques initially suffered from comparatively poor accuracy compared to short reads. However, with improvements in accuracy, accessibility, and cost efficiency, long-reads are gaining popularity in the field of scRNA-Seq.
Article Link: https://link.springer.com/article/10.1007/s00439-024-02678-x
Suitability of various tissues for scRNA‑Seq libraries preparation The choice of input tissue type suitable for scRNA-Seq depends on several factors, including the availability of adequate amounts of high-quality tissue, the goal of the experiment, and the method used for tissue preservation. Whole-cell scRNA-Seq approaches and single-nucleus RNA sequencing protocols (snRNA-Seq) are two widely used approaches of sc transcriptomics for various tissue types. Fresh-frozen tissue is often considered the gold standard for scRNA-Seq experiments as it provides the highest quality RNA. However, snRNA-Seq protocols can be applied to snap-frozen samples, avoiding many of the dissociation-related artifacts.
Bioinformatics tools and pipelines.
