Difference between proper paired reads and mapped and paired reads
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6.1 years ago

Hi,

I am using samtools stats to get some information about my genome. Can anybody explain to me the difference between reads mapped and paired and reads properly paired?

Thanks.

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6.1 years ago

reads mapped and paired

the input of the mapping was a set of two fastq files R1.fq and R2.fq from a paired end experiment

reads properly paired?

reads R1 and R2 from the same segment are mapped on the same chromosome, at a good expected distance with the correct directions. see https://www.slideshare.net/lindenb/ngsformats from slide 30.

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can you share the slide again?

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sorry, I deleted everything.

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