Exon count and GC content

Question

[Varsha]

I have a list of novel transcripts after filtering and also the gtf file and fasta file of these novel transcripts.. I also have their chromosome locations. How do I calculate their GC content and find out their exon count? How do I proceed with it? this is how my fasta file looks:

>NET.31408.1 loc:17|336737-341315|+ exons:336737-337034,338681-339015,339449-339828,341216-341315 segs:1-298,299-633,634-1013,1014-1113
CACAAACCACCCTTGCATTCTGCCAGCCACCCCTCTGCAAAACTGGTGCTCCAGCCACCAGGACCTTGAG
GCATTGCAGCCGCCCAGCCTTGTCTCCGGCACCCTCTCACCTGGAACGCCTTTGGTTCACGCTGTCTACC
TCCTCCACCTGGGGGTGGCCCAGCACCACCTCCCCTGGGAATTCTCCAGCTCCTCCCATCAGGCTCCCAT
TCGGCTTGAGCCCACCGCCCTCCCGTCAGCATTTCATTCCGCCCGCATCCTCGGGGGCATTTACCTGTTA
CCCCGATGCCCAGACATGAAACGCAGGCCTGCTTCCATTTACGTGATGTATGTGGGTCTGGAAAGTCCCA
GGCAGGCAGAATCTTTGCAGAGGAAACCTGATTTCGGCTCCCACCTGGGAACTGCTTGTTGAAGGAGCCC
AAGAGAAACCTCTCCATGAAGCAGAGAAGCTTCTAGGGAAAAAGAAGCCTCAACCCTCCTCACCCGCTTG
GAAAAGGCCCAGTCCTCAGGTGTGCTGAGGGCGGTGCTCCAGGCCCCGGGGGGCAGCGTCCCACACCCCT
GCCTCCGCCAGCAGCTTCTGCACGGCCCAGCCCAGACTCCAGCTCCCAGGTGGCTCTCCGCGGGTCCTGC
CAGCCTGACCCTGCACTACCAAACTGGGAGAGGAAGAAGCCGCCTCCATGGGTGCTGCCCACCTGCCAGG
TGCCCGCCACTGGCTGACCAACTGGAATCATCACAAGCCCCAGAGGACGACGTGATCATCACTCCTTTCA
GAAAAGAAGAAACCAGCTCGAGAGGGGCAGCCACGTGCCCAAGGCCCCATAAGCTGGCACCAGGTGCCCA
GTTTGGCCCAACGGAGCTGGGCTGAGCCCAGGTGCTTTCTATCCCCCTCCTCCTCCCAAGGCGTCGGGTT
GCAGGTGCGGTGCCTACAGGTGCCTAACGAAAGCAATGAGCCGGGTATTCTCCGAGCACCTGCCACACAC
CCAGCAGCGGGGAGCACAGAGTTCCCAGAAACTAACTTCGCAGTTTCTGGTGAACGTGTCTGACCTCTCC
TACTGGACCAAACACTCCCTCAGAGCAGGATGCCTCCTGCCCATATGGTACTGAAAACTGTGG
>NET.31408.2 loc:17|336737-342935|+ exons:336737-337034,338681-339015,342853-342935 segs:1-298,299-633,634-716
CACAAACCACCCTTGCATTCTGCCAGCCACCCCTCTGCAAAACTGGTGCTCCAGCCACCAGGACCTTGAG
GCATTGCAGCCGCCCAGCCTTGTCTCCGGCACCCTCTCACCTGGAACGCCTTTGGTTCACGCTGTCTACC
TCCTCCACCTGGGGGTGGCCCAGCACCACCTCCCCTGGGAATTCTCCAGCTCCTCCCATCAGGCTCCCAT
TCGGCTTGAGCCCACCGCCCTCCCGTCAGCATTTCATTCCGCCCGCATCCTCGGGGGCATTTACCTGTTA
CCCCGATGCCCAGACATGAAACGCAGGCCTGCTTCCATTTACGTGATGTATGTGGGTCTGGAAAGTCCCA
GGCAGGCAGAATCTTTGCAGAGGAAACCTGATTTCGGCTCCCACCTGGGAACTGCTTGTTGAAGGAGCCC
AAGAGAAACCTCTCCATGAAGCAGAGAAGCTTCTAGGGAAAAAGAAGCCTCAACCCTCCTCACCCGCTTG
GAAAAGGCCCAGTCCTCAGGTGTGCTGAGGGCGGTGCTCCAGGCCCCGGGGGGCAGCGTCCCACACCCCT
GCCTCCGCCAGCAGCTTCTGCACGGCCCAGCCCAGACTCCAGCTCCCAGGTGGCTCTCCGCGGGTCCTGC
CAGAGAATTTATAGAGTCTCATTGACCAACCAGCCAGACATGATGCTAATCTGGGTTCCAAAAACAAGAA
ACACCACGACAGATCA

and the GTF file:

17  StringTie   transcript  6670990 6676823 1000    -   .   gene_id "NET.31822"; transcript_id "NET.31822.1"; 
17  StringTie   exon    6670990 6671996 1000    -   .   gene_id "NET.31822"; transcript_id "NET.31822.1"; exon_number "1"; 
17  StringTie   exon    6676715 6676823 1000    -   .   gene_id "NET.31822"; transcript_id "NET.31822.1"; exon_number "2"; 
8   StringTie   transcript  140349489   140350371   1000    +   .   gene_id "NET.78699"; transcript_id "NET.78699.2"; 
8   StringTie   exon    140349489   140349957   1000    +   .   gene_id "NET.78699"; transcript_id "NET.78699.2"; exon_number "1"; 
8   StringTie   exon    140350234   140350371   1000    +   .   gene_id "NET.78699"; transcript_id "NET.78699.2"; exon_number "2"; 
3   StringTie   transcript  14136345    14137669    1000    +   .   gene_id "NET.53089"; transcript_id "NET.53089.5"; 
3   StringTie   exon    14136345    14136680    1000    +   .   gene_id "NET.53089"; transcript_id "NET.53089.5"; exon_number "1"; 
3   StringTie   exon    14137357    14137669    1000    +   .   gene_id "NET.53089"; transcript_id "NET.53089.5"; exon_number "2"; 
20  StringTie   transcript  58657036    58659388    1000    -   .   gene_id "NET.49267"; transcript_id "NET.49267.2"; 
20  StringTie   exon    58657036    58657089    1000    -   .   gene_id "NET.49267"; transcript_id "NET.49267.2"; exon_number "1"; 
20  StringTie   exon    58658947    58659388    1000    -   .   gene_id "NET.49267"; transcript_id "NET.49267.2"; exon_number "2"; 
8   StringTie   transcript  79826214    79827927    1000    -   .   gene_id "NET.77436"; transcript_id "NET.77436.1"; 
8   StringTie   exon    79826214    79826726    1000    -   .   gene_id "NET.77436"; transcript_id "NET.77436.1"; exon_number "1"; 
8   StringTie   exon    79827716    79827927    1000    -   .   gene_id "NET.77436"; transcript_id "NET.77436.1"; exon_number "2"; 
17  StringTie   transcript  336737  341315  1000    +   .   gene_id "NET.31408"; transcript_id "NET.31408.1"; 
17  StringTie   exon    336737  337034  1000    +   .   gene_id "NET.31408"; transcript_id "NET.31408.1"; exon_number "1"; 
17  StringTie   exon    338681  339015  1000    +   .   gene_id "NET.31408"; transcript_id "NET.31408.1"; exon_number "2"; 
17  StringTie   exon    339449  339828  1000    +   .   gene_id "NET.31408"; transcript_id "NET.31408.1"; exon_number "3"; 
17  StringTie   exon    341216  341315  1000    +   .   gene_id "NET.31408"; transcript_id "NET.31408.1"; exon_number "4"; 
3   StringTie   transcript  171240150   171244033   1000    +   .   gene_id "NET.56472"; transcript_id "NET.56472.1"; 
3   StringTie   exon    171240150   171240201   1000    +   .   gene_id "NET.56472"; transcript_id "NET.56472.1"; exon_number "1"; 
3   StringTie   exon    171243772   171244033   1000    +   .   gene_id "NET.56472"; transcript_id "NET.56472.1"; exon_number "2"; 
2   StringTie   transcript  8701295 8702421 1000    -   .   gene_id "NET.41416"; transcript_id "NET.41416.1"; 

[rfran010]

I have difficulty reading this (format lost in translation?), but unless your trying to do this programmatically as an exercise, I would just use the available tools, maybe seqkit, fastqc, subread, or bedtools?