Can I go from kallisto results to SEURAT directly

Question

[biotrekker]

Is there any thing else that needs to be done before I use SEURAT in R on my kallisto results. Am I missing a step. I heard about bustools but can't find more info on it. (Also this is single cell rna-seq data)

Thanks

[bk11]

If you already have matrix.mtx, genes.txt and barcodes.txt from kallisto output, you are ready to proceed into R for Seurat object creation.

library(Seurat)
?ReadMtx()

[biotrekker]

Is their anything kallisto bustools that should be done first that I am unaware of? Because I get the three files you are mentioning after kallisto quant?

[dsull]

Have you checked out the online tutorials? There are some older tutorials here: https://www.kallistobus.tools/ and a more recent one is here: https://www.biorxiv.org/content/10.1101/2023.11.21.568164v2.full.pdf

You'll need to show us exactly what your "kallisto results" are (i.e. what files you have) otherwise we have no idea what you're doing.