MGI DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0, Seurat

Question

[yau]

Dear All,

I would like to know if I can input MGI single cell rna-seq data into Seurat for analysis?

I only used 10X data for seurat before.

What will be the "filtered_feature_bc_matrix" which includes barcodes.tsv.gz, features.tsv.gz and matrix.mtx.gz in MGI dnbc4tools output?

thanks!

Answer 1

[bk11]

Did you check this link below where it show how Seurat or Scanpy can be used for downstream analyses from MGI dnbc4tools output?

https://github.com/MGI-tech-bioinformatics/DNBelab_C_Series_HT_scRNA-analysis-software/blob/version2.0/doc/io.md