hi all,
I have a question: I have several samples with quiagen quiaseq miRNA library UDI kit, sequenced on Illumina Novaseq 6000 (1x76bp).
When I check my quality fastq file, I obtained 2 adapter sequences shown in plot. (see the figure).
Why?
Can you help me?
Thank you for the information. What about trimming? Is it necessary to remove both adapters? Or just the 3' NGS adapter from the Qiagen kit?
Yes you will need to remove the adapters. Since the Illumina adapter follows the smRNA one it should be removed automatically. Follow the analysis directions included with the kit.