Entering edit mode
4 months ago
kayah
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20
Hello I want to know criterion of mitochondrial percent in scRNAseq I know that too high percentage means no good cell quality but i don't understand it's criterion.. If i analyze some tissue it contains variety of cells so I don't get which criterion suits for scRNAseq. If you know about this please tell me about it thank you!
See this post for some additional comments (on other QC metrics, but the same logic applies - blanket thresholding approaches have some drawbacks).
There are caveats. For example, cardiomyocytes can have very high MT gene expression.
This is definitely a very important and efficient filter I will definitely include in hands-on analysis; However, I am hesitating to integrate this into an automatic pipeline without checking the plot.