NetworkAnalyst Tool was used to determine and assess the genes that showed varying levels of expression between normal low density bone marrow cells and those from aplastic fanconi anemia based on statistically significant factors in database GSE16334. The set of genes were defined as having rows that reflect each gene entry and columns that indicate the specimens. BAsed on the experimental results, samples were distributed evenly between 11 wild types and 21 mutants, and all genes assay identities were converted to Entrez IDs. NetworkAnalyst tool, an integrated statistical analysis web application, was used for microarray meta-analysis. The variance stabilizing normalization (VSN) is a statistical method that uses a set of parametric transformations and maximum likelihood estimated to make sample variances independant of their mean intensities and bring the samples onto the same scale. Can someone explain to me what is the real meaning of this statement. how they use networkanalyst tool.
