I've setup a local version of PROVEAN (http://provean.jcvi.org/index.php) to run variant effect prediction and rank the severity of amino acid changing variants. I noticed that there is no examples of encoding stop codons for the input. PROVEAN uses one letter amino acid code for the input variant like "C671Y" and all such examples worked fine. But I did not find an example of encoding the stop codons. If there is a stop codon gain mutation, such as C671stop, I tried a few different encoding, C671stop, C671*, C671X, and none of them is valid according to PROVEAN. Or, a stop gain is server enough for any protein, PROVEAN would not process this type of mutation? Does anyone have any experience with this? Any suggestion would be really helpful!
Went through the HGVS standard page and tried the following: "Ter" and "*" for stop codon introduced by substitutions, and in both scenarios PROVEAN complained about "C671Ter: invalid format", "C671*: invalid format".