Entering edit mode
5 months ago
bioinfo
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150
Hello,
I have single cell data that I have analyzed using scanpy and I have assigned the cell types to my clusters. I want to run palantir for trajectory analysis but only for the neutrophils in my data and I am not sure at which point I should filter my andata object for the neutrophils. Is it appropriate to do it like this?
- Import raw data again
- Do QC
- Normalize
- Log
- Filter for the neutrophils
- PCA
- Diffusion maps etc
Thank you