How to run trajectory analysis with only one cell type using palantir?
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5 months ago
bioinfo ▴ 150

Hello,

I have single cell data that I have analyzed using scanpy and I have assigned the cell types to my clusters. I want to run palantir for trajectory analysis but only for the neutrophils in my data and I am not sure at which point I should filter my andata object for the neutrophils. Is it appropriate to do it like this?

  1. Import raw data again
  2. Do QC
  3. Normalize
  4. Log
  5. Filter for the neutrophils
  6. PCA
  7. Diffusion maps etc

Thank you

palantir scanpy • 190 views
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