Is the 10X multiome chemistry most similar similar to 10XV1, 10XV2, or 10XV3?

Question

[biotrekker]

Hello, I have some snRNAseq data that used 10x multiome chemistry. However, I am using kallisto and bustools via the kb wrapper. I need to specify the tech and the relevent ones are 10XV1, 10XV2, or 10XV3. Which one would 10X multiome be most similar too?

Thanks,

Answer 1

[dsull]

For 10X Multiome GEX?

According to https://kb.10xgenomics.com/hc/en-us/articles/360049373331-Can-Multiome-ATAC-and-Multiome-GEX-libraries-be-sequenced-together -- "Read Number 1 contains the 16-bp 10x barcode and 12-bp UMI, totalling 28 bases." -- This is 10XV3.

[biotrekker]

thank you!!!