I am getting an error with Kallisto Bustools wrapper (kb) command to create an index

Question

[biotrekker]

I am running the command: kb ref -i index.idx -g t2g.txt -f1 cdna.fasta genome.fasta genome.gtf

where genome.fasta= Homo_sapiens.GRCh38.cdna.all.fa.gz and genome.gtf= Homo_sapiens.GRCh38.112.gtf.gz

However, I am getting an error message (what should I do?):

Answer 1

[dsull]

Answered here: https://github.com/pachterlab/kb_python/issues/260

"You’re using the wrong fasta (.fa.gz) file — you need to supply the GENOME fasta. Look for the primary assembly on ensembl’s website."

No need to cross-post issues across GitHub+Biostars for kb-python related questions (because I routinely monitor both sites); wait 24-48 hours and if it doesn't get answered on one site, then post on the other.