Building a Kraken2 Database

Question

[Ahiad Chen Zion]

Hello BioStars Community,

I am in the process of setting up a Kraken2 database specifically for cress DNA viruses. While I have successfully downloaded the reference sequences manually, I am unable to use Kraken2’s automated tools to download the library. I am looking for advice on how to properly integrate these manually create kraken database using my reference

Downloaded Sequences: I have the reference sequences for cress DNA viruses ready.

Issue: The typical Kraken2 command for automatically downloading and adding sequences to the library doesn’t work in my case, and I'm unsure how to proceed with manually added sequences.

Thank you in advance for your support and suggestions!

Best,
Ahiad

[dthorbur]

Please provide the commands you are using and the errors in the output. We need to be able to see what you've tried and the errors you're getting to be able to provide accurate support.

[GenoMax]

If you have not found the necessary command to build your custom database then see --> https://github.com/DerrickWood/kraken2/issues/169