Technical Issue to building a database on snpEff for SNP calling annotation in Rice
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4 months ago
Brijesh • 0

Hi, I am currently working on building a database on snpEff, because the current database includes reference data Genome sequences [FASTA] IRGSP-1.0 genome (including organella and unanchored contig sequences), Gene annotation for snpEff [Annotation GTF] [Protein FASTA]RAP-DB (both representative and predicted genes) MSU (all genes in RGAP version 2). Also, I try GFF annotation fasta with Reference Genome fasta. I have already edited snpEff.config, end the end add Genome: 'RAP_MSU_on_IRGSP-1.0' However, I keep receiving the error below:

java -jar snpEff.jar build -gtf22 -v RAP_MSU_on_IRGSP-1.0
00:00:00 SnpEff version SnpEff 4.3t (build 2017-11-24 10:18), by Pablo Cingolani
00:00:00 Command: 'build'
00:00:00 Building database for 'RAP_MSU_on_IRGSP-1.0'
00:00:00 Reading configuration file 'snpEff.config'. Genome: 'RAP_MSU_on_IRGSP-1.0'
00:00:00 Reading config file:/home/VcfFiles/snpEff/snpEff.config
java.lang.RuntimeException: Property: 'RAP_MSU_on_IRGSP-1.0' not found
at org.snpeff.interval.Genome.<init>(Genome.java:106)
at org.snpeff.snpEffect.Config.readGenomeConfig(Config.java:681)
at org.snpeff.snpEffect.Config.readConfig(Config.java:649)
at org.snpeff.snpEffect.Config.init(Config.java:480)
at org.snpeff.snpEffect.Config.<init>(Config.java:117)
at org.snpeff.SnpEff.loadConfig(SnpEff.java:451)
at org.snpeff.snpEffect.commandLine.SnpEffCmdBuild.run(SnpEffCmdBuild.java:364)
at org.snpeff.SnpEff.run(SnpEff.java:1183)
at org.snpeff.SnpEff.main(SnpEff.java:162)
00:00:00 Logging
00:00:01 Done.
snpEff SNP • 240 views
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Entering edit mode

what is the output of

grep -C 10 -F 'RAP_MSU_on_IRGSP-1.0'  /home/VcfFiles/snpEff/snpEff.config
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