Technical Issue to building a database on snpEff for SNP calling annotation in Rice
0
0
Entering edit mode
3 months ago
Brijesh • 0

Hi, I am currently working on building a database on snpEff, because the current database includes reference data Genome sequences [FASTA] IRGSP-1.0 genome (including organella and unanchored contig sequences), Gene annotation for snpEff [Annotation GTF] [Protein FASTA]RAP-DB (both representative and predicted genes) MSU (all genes in RGAP version 2). Also, I try GFF annotation fasta with Reference Genome fasta. I have already edited snpEff.config, end the end add Genome: 'RAP_MSU_on_IRGSP-1.0' However, I keep receiving the error below:

java -jar snpEff.jar build -gtf22 -v RAP_MSU_on_IRGSP-1.0
00:00:00 SnpEff version SnpEff 4.3t (build 2017-11-24 10:18), by Pablo Cingolani
00:00:00 Command: 'build'
00:00:00 Building database for 'RAP_MSU_on_IRGSP-1.0'
00:00:00 Reading configuration file 'snpEff.config'. Genome: 'RAP_MSU_on_IRGSP-1.0'
00:00:00 Reading config file:/home/VcfFiles/snpEff/snpEff.config
java.lang.RuntimeException: Property: 'RAP_MSU_on_IRGSP-1.0' not found
at org.snpeff.interval.Genome.<init>(Genome.java:106)
at org.snpeff.snpEffect.Config.readGenomeConfig(Config.java:681)
at org.snpeff.snpEffect.Config.readConfig(Config.java:649)
at org.snpeff.snpEffect.Config.init(Config.java:480)
at org.snpeff.snpEffect.Config.<init>(Config.java:117)
at org.snpeff.SnpEff.loadConfig(SnpEff.java:451)
at org.snpeff.snpEffect.commandLine.SnpEffCmdBuild.run(SnpEffCmdBuild.java:364)
at org.snpeff.SnpEff.run(SnpEff.java:1183)
at org.snpeff.SnpEff.main(SnpEff.java:162)
00:00:00 Logging
00:00:01 Done.
snpEff SNP • 239 views
ADD COMMENT
0
Entering edit mode

what is the output of

grep -C 10 -F 'RAP_MSU_on_IRGSP-1.0'  /home/VcfFiles/snpEff/snpEff.config
ADD REPLY

Login before adding your answer.

Traffic: 1604 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6