Entering edit mode
3 months ago
kyusikkim
▴
20
Hello all, I am trying to use the wf-transcriptomes pipeline for nanopore sequencing data from S. cerevisiae. However, StringTie likes to merge UTR regions with nearby transcripts, thus creating erroneous and extremely long transcripts that do not make sense. Does anyone have any suggestions for parameters when using StringTie --merge or when using StringTie to construct transcripts?