qPRO-Seq analysis

Question

[daffodil]

Hi everyone,

I'm currently working on qPRO-Seq analysis and need to normalize my data using spike-in reads. I understand that normalization is crucial to account for technical variations and ensure accurate comparisons across samples. However, I'm not entirely sure about the best method to calculate normalization factors based on the spike-in reads.

Could someone provide a detailed explanation or point me towards resources or tools that can help with this specific type of normalization in qPRO-Seq analysis? Any examples or step-by-step guides would be greatly appreciated!

Thanks in advance for your help!