I'm very new to bioinformatics and i was trying to run a FastQC excersice. I was running the SRR074262 run from the NCBI SRR database, but my QC rapport does not give me any yellow boxplots on the per base sequence quality. This should not be the case. Can anyone eplain what I'm doing wrong?
The absence of yellow boxplots in your FastQC report indicates that your data is very clean. In this case, even the 10th percentiles do not drop below the quality score threshold (around 30), which is why you are seeing consistent, high-quality scores.
If you want to take an additional step to verify your results, you can run your data through another quality control tool like Fastp. You should observe similar high-quality metrics, confirming that your data is indeed of exceptional quality.
Keep up the good work in bioinformatics!
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version 2.3.6
This has been asked many times before, e.g. Fastq file with very high per base sequence quality and no box-whisker plot