BUSCO analysis error

Question

[Shakunthala Natarajan]

I installed BUSCO v5.7.1 using Conda and ran the following command:

./bin/busco -i abc_genomic.fna --out XYZ --out_path /path_to_output_folder/  -m genome --auto-lineage &

However, I am encountering an error as follows:

 busco.Exceptions.BatchFatalError: [Errno 5] Input/output error

It would be great if someone could help me in resolving this. Thank you!

[Mensur Dlakic]

If you installed BUSCO using conda, you should run it simply with busco rather than ./bin/busco. A directory specified as output path must be writeable by you. What you specified (/path_to_output_folder/) does not meet that condition.

[Shakunthala Natarajan]

I ran busco from outside the bin folder, hence I used ./bin/busco to execute it. Thank you for pointing out the folder permission. But even after changing the permissions, I was unable to resolve the error. I then found that there are two versions of busco installed, perhaps this could also be causing the error. I plan to uninstall the old version and run the analysis again. Thank you for helping out though!