Entering edit mode
4 months ago
pixie@bioinfo
★
1.5k
Hello, I am trying to map ONT reads against the latest NCBI database which consists of plant reads + viruses. The database is huge and I get this error:
Command:
-a -x map-ont -y -t 60 --split-prefix tmp_minimap
Error:
minimap2: hit.c:210: mm_hit_sort: Assertion 'has_cigar + no_cigar == 1' failed
KIndly help,
Thanks
Did you pre-create the database or are you supplying the fasta as you are mapping?
I pre-created the database and mapping is done as a part of automated pipeline