What tool is best to build plasmid using a reference sequence?
0
0
Entering edit mode
3 months ago
blur ▴ 280

Hi,

I have a plasmid assembled using both short and long reads.

Also, I have other WGS samples, sequenced before using illumina short reads that contain the same plasmid

I want to use the assembled plasmid as a reference/scaffold to build upon.

Can anyone direct me to the right tool? Is there such an option in Unicycler?

Also - is the right path using an aligner like BWA or bowtie2?

assembly reference WGS • 356 views
ADD COMMENT
2
Entering edit mode

Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you should mark it as accepted. You can accept more than one answer if they all work. If an answer was not really helpful or did not work, provide detailed feedback so others know not to use that answer.

Upvote|Bookmark|Accept

ADD REPLY
0
Entering edit mode

After reading some more - should I map to reference with an aligner like BWA? or is there an assembly tool that uses a reference?

ADD REPLY

Login before adding your answer.

Traffic: 2729 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6