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3 months ago
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Hi,
I have a plasmid assembled using both short and long reads.
Also, I have other WGS samples, sequenced before using illumina short reads that contain the same plasmid
I want to use the assembled plasmid as a reference/scaffold to build upon.
Can anyone direct me to the right tool? Is there such an option in Unicycler?
Also - is the right path using an aligner like BWA or bowtie2?
After reading some more - should I map to reference with an aligner like BWA? or is there an assembly tool that uses a reference?