I am working on a deconvolution analysis using the CIBERSORT algorithm, which typically requires a signature matrix (sig_matrix_file.txt) with continuous gene expression values for different cell types.
My question is:
Is it possible to use a binary signature matrix (containing only 0s and 1s) instead of continuous values in CIBERSORT? For example, where a '1' indicates the presence and a '0' indicates the absence of gene expression in a given cell type.
Would using a binary matrix impact the accuracy of the deconvolution? Are there any modifications needed in the CIBERSORT algorithm to accommodate a binary matrix? Has anyone successfully used a binary matrix with CIBERSORT, or is it strictly recommended to use continuous values? I appreciate any insights or experiences you can share on this matter!
Thank you.
