Affymetrix microarray
1
0
Entering edit mode
4 weeks ago

How to do one-to-one mapping between gene and probe set with Affymetrix mircoarray data? How to select the best probe value for each gene? or How to summarize multiple probes values of one gene into one representative value?

Affymetrix microarray data

gene-expression probes microarray • 432 views
ADD COMMENT
1
Entering edit mode

If you want to learn about microarray analysis, you should look into Bioconductor from R. There are a lot of packages and workflows like arrays.

ADD REPLY
0
Entering edit mode

If you look at the platform entry for the array at NCBI site you should be able to find the annotation for the array. Looks like you are working with https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL96

Take a look at the data table.

ADD REPLY
0
Entering edit mode
20 days ago

You can follow my guidance, here: How do I convert Affymetrix ID names to gene names

You will have to modify the above code depending on which Affymetrix array that you are using. It looks like you are using the U133A.

Note that you can summarise expression values across probes targeting the same gene via the limma::avereps function - How to combine expression values of multiple probes for one gene?

Kevin

ADD COMMENT

Login before adding your answer.

Traffic: 1425 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6