Hi all
I have protein sequences downloaded from the uniprotkb's swissprot. I would like to functionally encode the amino acid residues which are considered to be catalytic in nature and annotated in the database. Currently, I have used HMMs and Pfam-A to encode those domains that are returned as significant. Since this method is more toward functional annotation prediction and determining which regions are significant based on an E-value, I would very much like to encode those amino acid residues which are experimentally annotated as catalytic in nature.
I have done the following for now: I looked at two proteins: PO7327 and Q6PHW0. The former has annotated catalytic regions. The latter does not even though they both have experimentally annotated EC numbers.
I was hoping to know if there are any resources which details the catalytic sites of the enzymes that are present in the uniprot's swissprot database?
Thanks in advance and any help is really appreciated.
