IGV and display of amino acid translations

Question

[sk]

Hello, I don't understand why when I right-click -> translate (in IGV), I see 3 different amino acid sequences appear.

• Why are there 3 different sequences?
• Why isn't RefSeq consistent with the displayed sequences?

Answer 1

[alex.zaccaron]

Looks like IGV is showing all 3 possible translation frames on the forward strand, and your Refseq Gene in on the reverse strand. Right-click -> flip strand should display what you want.

[sk]

Thanks, it works

[Ram]

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