Sorting DEGs in the dotplot for every column

Question

[ashkan]

I am trying to sort the genes (blue and grey dots) in each column in my dotplot and put the blue ones together and grey one together in each column (which means per sample). what argument should I add to the following command? the dotplot is made for DEGs between conditions.

DotPlot(mydata, features = rev(as.character(unique(top20_sub$gene))), group.by = "orig.ident", assay = "RNA") + coord_flip() + RotatedAxis()

Answer 1

[Bastien Hervé]

If you are looking for something like a hierarchical clustering of your genes in the DotPlot function, seems like the feature does not exist (question asked here). The only thing you can cluster is your identities.

A work around would be to generate the DotPlot, extract the data, reorganize them the way you want and replot.

dp <- DotPlot(mydata, features = rev(as.character(unique(top20_sub$gene))), group.by = "orig.ident", assay = "RNA") + coord_flip() + RotatedAxis()
dp$data