Heatmap for DEGs

Question

[4732b159]

hello, I have computed DEG analysis on RNA seq data and for visualization, is it better to plot some sig genes or only top sig up regulated genes in heatmap? Thank you for insights!

[jeevii]

hello,

I would suggest to go ahead with top-20/30 up and down regulated genes while considering p-significance as well.

hope it helps!

[4732b159]

thanks for your reply :) do you mean you filter up and downs together and plot them together in the same heatmap? sorry for this naive question, trying to learn more..

Answer 1

[jeevii]

yes, it will clearly show the gene regulation between your control and treatment conditions.

[4732b159]

thanks!