reconstruct ecDNA.

Question

[María José]

Hi,

I'm new in the field of Extrachromosomal DNA (ecDNA) and I would appreciate any recommendations or advices for software tools designed to reconstruct human ecDNA derived from third generation sequencing data. Additionally, I would find very helpulf any websites detailing the typical steps involved in these reconstructions.

Our data consist of pacbio readings which have been aligned with pbmm2.

[Pierre Lindenbaum]

https://nf-co.re/circdna/1.1.0/

Pipeline for the identification of extrachromosomal circular DNA (ecDNA) from Circle-seq, WGS, and ATAC-seq data that were generated from cancer and other eukaryotic cells.