integrate two already processed 10x multimodal objects - problem
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12 weeks ago
Sky ▴ 10

Hello, I am having trouble integrating two multimodal datasets. For both datasets, RNA and ATAC-sequencing was performed in the same cell.

I ran the code below after I followed the https://stuartlab.org/signac/articles/pbmc_multiomic for each dataset:

multimodal.dataset1 <- FindMultiModalNeighbors(multimodal.dataset1, reduction.list = list("pca", "lsi"),
                                        dims.list = list(1:50, 2:50)) #contains RNA and ATAC sequencing for dataset1

multimodal.dataset2 <- FindMultiModalNeighbors(multimodal.dataset2, reduction.list = list("pca", "lsi"),
                                          dims.list = list(1:50, 2:50)) #contains RNA and ATAC sequencing for dataset2

multimodal.dataset1 <- RunUMAP(multimodal.dataset1, nn.name = "weighted.nn", reduction.name = "wnn.umap", reduction.key = "wnnUMAP_")
multimodal.dataset1 <- FindClusters(multimodal.dataset1, graph.name = "wsnn", algorithm = 3, verbose = FALSE)

multimodal.dataset2 <- RunUMAP(multimodal.dataset2, nn.name = "weighted.nn", reduction.name = "wnn.umap", reduction.key = "wnnUMAP_")
multimodal.dataset2 <- FindClusters(multimodal.dataset2, graph.name = "wsnn", algorithm = 3, verbose = FALSE)

I then want to combine multimodal.dataset1 and multimodal.dataset2. Is there a way to do this?

multiome • 137 views
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