Forum:What companies do you recommend for targeted sequencing?

Question

[amy__]

Hi all,

I have a gene that we are looking at sequencing in a couple of hundred patients. Do any of you have recommendations for companies that do high quality targeted sequencing? Either for a single gene or for multiple. I want to make sure that the coverage is excellent as I can't risk having bad quality data.

Thanks,
Amy

Answer 1

[GenoMax]

Check with the usual suspects. You could either make up a custom panel or use one of the offered panels.

GeneWiz: https://www.genewiz.com/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels/
Eurofins: https://eurofinsgenomics.eu/en/eurofins-genomics/material-and-methods/targeted-sequencing/
Novogene: https://www.novogene.com/amea-en/services/genomics/target-capture-sequencing/
Revvity: https://www.revvity.com/in-en/category/targeted-sequencing
Paragon Genomics: https://www.paragongenomics.com/targeted-sequencing/

Or use one of the kits in your own lab:

Twist: https://www.twistbioscience.com/products/ngs
Illumina: https://sapac.illumina.com/techniques/sequencing/dna-sequencing/targeted-resequencing/targeted-panels.html
IDT: https://www.idtdna.com/pages/technology/next-generation-sequencing/dna-sequencing/targeted-sequencing

[amy__]

Thanks! I've reached out to a few so I'll see what they say!

Answer 2

[Jeremy]

We've found that Psomagen has fewer sequencing errors compared to Azenta/GeneWiz: Psomagen Targeted Sequencing

[amy__]

Oo thank you, I'll have a look into them!