Tools for assembling Plasmid Genome
0
0
Entering edit mode
3 months ago
adarsh_munna ▴ 50

Hi,

I have three illumina-sequenced plasmid samples, which should be assembled. I have triend assembly with normal SPAdes and also with plasmidSPAdes. plasmidSPAdes gave assembly for only one sample, whereas SPAdes gave assembly for all the samples. However I am unable to obtain the required output. The expected plasmid size is about 3 to 10 kb.

Are there any other tool for doing the process?

Please suggest.

Thanks

Illumina Plasmid E.coli Assembly • 322 views
ADD COMMENT
0
Entering edit mode

However I am unable to obtain the required output.

And that output is?

ADD REPLY
0
Entering edit mode

Hi, I am expecting an assembly with required genome size. But, I am getting too many contigs with smaller size and the BLAST is hitting against different genomes.

ADD REPLY

Login before adding your answer.

Traffic: 1742 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6