lacking reference transcriptome

Question

[Sarah]

I am an undergraduate student who is under a professor's research regarding transcriptomic analysis of Abrus precatorius, but now my progress is stuck because of the lacking reference data for enrichment. Even when I wanted to match it with Arabidopsis thaliana as an ortholog, the enrichment is still unavailable. Has anyone gone through this problem with a novel species? Thank you in advance.

[GenoMax]

reference data for enrichment.

What does that mean? A genome reference is available for Abrus precatorius from NCBI: https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/003/935/025/GCF_003935025.1_Abrus_2018/

Take a look at the README.txt file. You will likely want to use https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/003/935/025/GCF_003935025.1_Abrus_2018/GCF_003935025.1_Abrus_2018_cds_from_genomic.fna.gz as the transcriptome.

[ATpoint]

I am an undergraduate student who is under a professor's

Talk to your supervisor and ask for guidance. If you need strangers on the internet for such basic tasks and your group has no support for you I would reconsider whether spending time with this group is a good investment of time. Saying this in your best interest. Undergraduates should follow some guided research efforts, not figuring it all out themselves. Lots of time for that in a PhD.

[colindaven]

Are you trying to do RNA-seq analysis ? Do you have RNA-seq reads from Illumina in fastq format ?