Facing problems in analysis of bisulfite sequencing data
0
0
Entering edit mode
10 weeks ago
SHREYA • 0

Facing a lot of problem in analysing the bisulfite seqencing data, its not aligning with (bioinfomatically bisulfite converted) reference sequence. I can't identify the signature sequences so as to identify the CpG sites. Only reverse primer sequence is matching in all the clone sequencing data but the Guanines (G) are converted to Adenines (A).. Is it possible? I am very confused as usually I know that C is converted to T.. I have performed Sanger Sequencing. Can anyone please help me in this regards????

sequencing Bisulfite • 218 views
ADD COMMENT

Login before adding your answer.

Traffic: 1896 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6