Why are the SINEs annotated as zero when using RepeatModeler + RepeatMasker?
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5 weeks ago
mut • 0

When I use the RepeatModeler and RepeatMasker pipeline to annotate repeats in a plant genome, the results show that SINEs are 0. However, when I used EDTA, it detected SINEs. How can I solve this problem? I found that there are no SINEs in the RepeatModeler result file XX.families.fa. What does this indicate?enter image description here

SINEs Repeatmodeler RepeatMasker • 315 views
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Entering edit mode
5 weeks ago
Michael 55k

In short, this means they haven't been detected by RepeatModeler. That is because correct detection of SINE's is much harder than of other TEs. EDTA is a different pipeline (but includes RepeatModeler) that has been developed and parameter-tuned to outperform other such pipelines. It is therefore not surprising that it yields slightly different results. You could integrate the SINE sequences into your repeat library, but expect many erroneous classifications. The EDTA paper has more background on this and also elaborates on the areas with room for improvement (including SINE detection).

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