why do I get this error while reading single cell RNA seq data in R

Question

[nuorain]

Hi, I got this error. Why? I appreciate your help. Thank you very much.

Error in Read10X(data.dir = data_dir) : 
  Barcode file missing. Expecting barcodes.tsv.gz

list.files(data_dir)
 [1] "GSM5004180_PT1_barcodes.tsv.gz" "GSM5004180_PT1_features.tsv.gz"
 [3] "GSM5004180_PT1_matrix.mtx.gz"   "GSM5004181_PT2_barcodes.tsv.gz"

cyto<- Read10X(data.dir = data_dir) 
Error in Read10X(data.dir = data_dir) : 
  Barcode file missing. Expecting barcodes.tsv.gz

[GenoMax]

It is often useful to do a quick search with the error. Here is one of the first search results that explains the issue: https://github.com/satijalab/seurat/issues/1625 . YOu also have more than one sample in this folder.

Answer 1

[ATpoint]

What GenoMax links. These functions assume that files are called matrix.mtx.gt, barcodes.tsv.gz and genes.tsv.gz. That means, with multiple samples you need to create separate folders, with one set of files each.