genome annotation and GFF file formation
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8 hours ago

I have predicted some organism's whole genome annotation and produced gff files by using Meta Euk. but these gff files are not recognizable by feature count.

I am getting error like below

||                                                                            ||
|| Load annotation file dataset_fd73837e-381d-4b9f-bd69-503c68d90c19.dat ...  ||

ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file.
The specified gene identifier attribute is 'gene_id ' 
An example of attributes included in your GTF annotation is 'gene_id XP_049336233.1'.

my file looks like below

CM033892.1  gene    MetaEuk 54523435    54530177    656 +   .   gene_id "XP_049336233.1"; transcript_id "XP_049336233.1"; protein_name "XP_049336233.1 uncharacterized protein C14orf93-like [Astyanax mexicanus]";

Can any one help me to figure out the issue in the gff format? (I am using usegalaxy for differential gene expression analysis )

MetaEuk GFF whole-genome-annotation • 54 views
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