Entering edit mode
8 hours ago
manaswiniparija3
▴
40
I have predicted some organism's whole genome annotation and produced gff files by using Meta Euk. but these gff files are not recognizable by feature count.
I am getting error like below
|| ||
|| Load annotation file dataset_fd73837e-381d-4b9f-bd69-503c68d90c19.dat ... ||
ERROR: failed to find the gene identifier attribute in the 9th column of the provided GTF file.
The specified gene identifier attribute is 'gene_id '
An example of attributes included in your GTF annotation is 'gene_id XP_049336233.1'.
my file looks like below
CM033892.1 gene MetaEuk 54523435 54530177 656 + . gene_id "XP_049336233.1"; transcript_id "XP_049336233.1"; protein_name "XP_049336233.1 uncharacterized protein C14orf93-like [Astyanax mexicanus]";
Can any one help me to figure out the issue in the gff format? (I am using usegalaxy for differential gene expression analysis )